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How does the surveyor mutation detection kit for standard gel electrophoresis work?

The SURVEYOR Mutation Detection Kit for Standard Gel Electrophoresis has been designed to cleave unlabeled DNA fragments at mismatched sites for subsequent analysis by agarose gel electrophoresis or polyacrylamide gel electrophoresis (PAGE). DNA 200 to 4,000 bp long can be analyzed using manual agarose gel electrophoresis while smal... Read more

What is a DNA mutation detection kit?

SURVEYOR Mutation Detection Kits provide a simple, accurate and cost-effective means to scan DNA fragments for mutations with unmatched sensitivity and specificity.

What is included in the guide-it mutation detection kit?

The Guide-it Mutation Detection Kit contains all the reagents needed for PCR-based identification of insertions or deletions generated in the genome of cells treated with engineered nucleases such as zinc-finger nucleases (ZFNs), transcription-activator-like effector nucleases (TALENs), or CRISPR/Cas9.

What is Surveyor nuclease?

The key component of the kits is Surveyor Nuclease, a member of the CEL family of mismatch-specific nucleases derived from celery. Surveyor Nuclease recognizes and cleaves mismatches due to the presence of single nucleotide polymorphisms (SNPs) or small insertions or deletions.

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