Keyword Analysis & Research: streptomyces colony pcr

openwetware.org

https://openwetware.org/wiki/Streptomyces:Protocols/PCR

Nov 19, 2009 · Polymerase Chain Reaction (PCR) is a method of amplifying a specific DNA target sequence. The cycle involves denaturing the template double stranded DNA (dsDNA) to single stranded DNA (ssDNA). Custom designed primers/oligonucleotides then anneal to their target sequence on the ssDNA. The DNA polymerase then extends the primers in a 5’ → 3’ …

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nih.gov

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC149868/

Feb 18, 2003 · Streptomycetes are high G+C Gram-positive, antibiotic-producing, mycelial soil bacteria. The 8.7-Mb Streptomyces coelicolor genome was previously sequenced by using an ordered library of Supercos-1 clones. Here, we describe an efficient procedure for creating precise gene replacements in the cosmid clones by using PCR targeting and λ-Red-mediated … Author: Bertolt Gust, Greg L. Challis, Kay Fowler, Kay Fowler, Tobias Kieser, Keith F. Chater Publish Year: 2003

Author: Bertolt Gust, Greg L. Challis, Kay Fowler, Kay Fowler, Tobias Kieser, Keith F. Chater

Publish Year: 2003

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sciencedirect.com

https://www.sciencedirect.com/science/article/pii/S016770120200235X

Jun 01, 2003 · Streptomycetes are attractive microorganisms because of their high secretion capacity and for the production of secondary metabolites. We report the improvement of a PCR-based method for screening of solid media-grown Streptomyces transformants. By resuspending mycelium into 2.5–10% dimethyl sulfoxide (DMSO), extensive manipulation prior to PCR could … Author: W. Van Dessel, L. Van Mellaert, N. Geukens, J. Anné Publish Year: 2003

Author: W. Van Dessel, L. Van Mellaert, N. Geukens, J. Anné

Publish Year: 2003

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researchgate.net

https://www.researchgate.net/post/I_need_easy_method_to_extract_DNA_from_streptomyces_except_bead_method_or_authetic_method_for_direct_colony_PCR_from_streptomyces_with_details

It is best to use very fresh mycelia in PCR reactions, older usually does not work; older spores generally work fine though. In our experience a strain typically works well for colony PCR or is...

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neb.com

https://www.neb.com/applications/cloning-and-synthetic-biology/dna-analysis/colony-pcr

Colony PCR is a convenient high-throughput method for determining the presence or absence of insert DNA in plasmid constructs. Individual transformants can either be lysed in water with a short heating step or added …

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nih.gov

https://pubmed.ncbi.nlm.nih.gov/21053253/

This unit includes general protocols for the genetic manipulation of Streptomyces species, including genomic DNA isolation, genomic library preparation, intergeneric conjugation of Streptomyces with E. coli, generation and transformation of Streptomyces protoplasts, electroporation of Streptomyces mycelia, and colony PCR. Publication types

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researchgate.net

https://www.researchgate.net/post/What-is-the-best-method-for-extraction-of-genomic-DNA-from-Streptomyces-spp-for-PCR

For routine Streptomyces PCR, my lab does not extract DNA. It's laborious and time consuming, especially if you have many strains to check. Instead, try …

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