https://en.wikipedia.org/wiki/RNA-Seq
WebRNA-Seq (named as an abbreviation of RNA sequencing) is a technique that uses next-generation sequencing to reveal the presence and quantity of RNA molecules in a biological sample, providing a snapshot of gene expression in the sample, also known as transcriptome.
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2949280/
WebRNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods.
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4863231/
WebAbstract. RNA sequencing (RNA-Seq) uses the capabilities of high-throughput sequencing methods to provide insight into the transcriptome of a cell. Compared to previous Sanger sequencing- and microarray-based methods, RNA-Seq provides far higher coverage and greater resolution of the dynamic nature of the transcriptome.
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https://www.nature.com/articles/s41576-019-0150-2
WebJul 24, 2019 · Over the past decade, RNA sequencing (RNA-seq) has become an indispensable tool for transcriptome-wide analysis of differential gene expression and differential splicing of mRNAs.
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https://www.nature.com/articles/nrg2484
WebThis article explains how RNA-Seq works, the challenges it faces and how it is changing our view of eukaryotic transcriptomes. RNA-Seq is a recently developed approach to transcriptome...
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https://www.technologynetworks.com/genomics/articles/rna-seq-basics-applications-and-protocol-299461
WebApr 6, 2018 · RNA-seq (RNA-sequencing) is a technique that can examine the quantity and sequences of RNA in a sample using next-generation sequencing (NGS). Here, we look at why RNA-seq is useful, how the technique works, a basic protocol that is commonly used today and challenges that remain.
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6096346/
WebDec 14, 2017 · Our goals in the present review are to break down the steps of a typical RNA-seq analysis and to highlight the pitfalls and checkpoints along the way that are vital for bench scientists and biomedical researchers performing experiments that use RNA-seq.
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https://www.nature.com/articles/s41368-021-00146-0
WebNov 15, 2021 · Bulk RNAseq. Since the first EST library was sequenced using Roche 454 sequencer in 2007 1, bulk RNAseq has become the most valuable and extensively used tool in understanding cancer biology. Its ...
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https://www.illumina.com/techniques/sequencing/rna-sequencing.html
WebBenefits of RNA Sequencing. RNA-Seq with next-generation sequencing (NGS) is increasingly the method of choice for scientists studying the transcriptome. Covers an extremely broad dynamic range. Provides sensitive, …
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https://www.youtube.com/watch?v=8lAVfKbRK3I
WebAre you looking for deeper insight into the transcriptome? RNA Sequencing is quickly become the gold standard for studying gene expression. Now is the time t...
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https://www.nature.com/subjects/rna-sequencing
WebApr 11, 2024 · RNA sequencing refers to techniques used to determine the sequence of RNA molecules. It includes high-throughput shotgun sequencing of cDNA molecules obtained by reverse transcription from RNA ...
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https://genomemedicine.biomedcentral.com/articles/10.1186/s13073-017-0467-4
WebAug 18, 2017 · RNA sequencing (RNA-seq) is a genomic approach for the detection and quantitative analysis of messenger RNA molecules in a biological sample and is useful for studying cellular responses. RNA-seq has fueled much discovery and innovation in medicine over recent years.
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https://mbernste.github.io/posts/rna_seq_basics/
WebJan 7, 2021 · Overview of RNA-seq. Here are the general steps of an RNA-seq experiment: Isolation: Isolate RNA molecules from a cell or population of cells. Fragmentation: Break RNA molecules into fragments (on the order of a few hundred bases long). Reverse transcription: Reverse transcribe the RNA into DNA.
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https://www.sciencedirect.com/topics/neuroscience/rna-seq
WebRNA-Seq. RNA-seq refers to the combination of high-throughput next generation sequencing (NGS) with computational method to capture and quantify transcripts present in an RNA extract (Ozsolak and Milos, 2011). From: Encyclopedia of Bioinformatics and Computational Biology, 2019. Related terms: Microarrays; Transcriptome; Long Non-Coding …
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https://bitesizebio.com/13542/what-everyone-should-know-about-rna-seq/
WebJul 9, 2016 · RNA-seq lets you quantify, discover and profile RNAs. For this technique, mRNA (and other RNAs) are first converted to cDNA. The cDNA is then used as the input for a next-generation sequencing library preparation. In this article, I’ll give a brief review of RNA-seq and introduce the major methods being used today.
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https://www.cgm.northwestern.edu/cores/nuseq/services/next-generation-sequencing/rna-seq.html
WebRNA sequencing, or RNA-Seq, is the latest technology to study the transcriptome, i.e., the full set of RNA transcripts as genome readouts in a cell or population of cells. This technology directly sequences RNA molecules in the transcriptome in order to determine their genes of origin and abundance. RNA species need to undergo a sequencing ...
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https://pubmed.ncbi.nlm.nih.gov/31341269/
WebOver the past decade, RNA sequencing (RNA-seq) has become an indispensable tool for transcriptome-wide analysis of differential gene expression and differential splicing of mRNAs. However, as next-generation sequencing technologies have developed, so too has RNA-seq. Now, RNA-seq methods are available for studying many different aspects of RNA ...
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https://pubmed.ncbi.nlm.nih.gov/27198714/
WebIn this study, we review current RNA-Seq methods for general analysis of gene expression and several specific applications, including isoform and gene fusion detection, digital gene expression profiling, targeted sequencing and single-cell analysis.
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https://www.nature.com/articles/nrg2934
WebDec 30, 2010 · Recent developments in methods for RNA sequencing have led to an increased understanding of transcriptomes — both qualitative and quantitative. Ongoing developments include advances in direct ...
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https://pubmed.ncbi.nlm.nih.gov/19015660/
WebRNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts ….
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10043755/
WebMar 13, 2023 · Overview of RNA-seq. RNA-seq is a process of creating short sequencing reads from RNA molecules. The steps consist of first converting the RNA (A) into cDNA (B), then (optionally) amplifying the cDNA by PCR (C), and finally fragmenting the cDNA into short pieces (known as fragments).
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https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-024-10322-1
Web1 day ago · In recent years, Single-cell RNA sequencing (scRNA-seq) is increasingly accessible to researchers of many fields. However, interpreting its data demands proficiency in multiple programming languages and bioinformatic skills, which limited researchers, without such expertise, exploring information from scRNA-seq data. Therefore, there is a …
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https://bioconductor.org/packages/devel/bioc/vignettes/DESeq2/inst/doc/DESeq2.html
Web6 days ago · As input, the DESeq2 package expects count data as obtained, e.g., from RNA-seq or another high-throughput sequencing experiment, in the form of a matrix of integer values. The value in the i -th row and the j -th column of the matrix tells how many reads can be assigned to gene i in sample j.
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https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-024-10298-y
Web2 days ago · RNA-seq is considered to be a reliable tool to gain insight into the transcriptome [17,18,19,20]. Gong et al. investigated the developing mouse testes at the transcriptional level in pups (6 days postnatal), juveniles (4 weeks old), and adults (10 weeks old), but the study mainly focused on mRNA changes, and the roles that small RNAs may play ...
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https://www.nature.com/articles/s41598-020-76881-x
WebNov 12, 2020 · RNA-seq is currently considered the most powerful, robust and adaptable technique for measuring gene expression and transcription activation at genome-wide level.
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6168365/
WebRNA-seq involves isolation of total RNA from tissues or cells of interest followed by the construction of DNA libraries and sequencing of these libraries using a next-generation sequencing instrument. This Unit covers a basic computational workflow of bioinformatics analysis of RNA-seq data.
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https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1415915/full
Web3 days ago · In the published article, a reference was missing for the section: “We downloaded the sepsis single-cell sequencing sample GSE167363 from the GEO database and processed the data using the Seurat package, retaining cells with less than 10% mitochondrial genes, cells with a total number of genes ...
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https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1310376/full
Web2 days ago · We analyzed bulk RNA-seq data from >500 head and neck squamous cell carcinoma (HNSCC) samples profiled by The Cancer Genome Atlas (TCGA) using GEPIA2 and performed Kaplan-Meier analysis. Patients were divided into high and low expression groups based on median gene expression; the top 50% were high expression, and the bottom 50% …
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https://www.mdpi.com/2079-7737/13/5/283
Web2 days ago · This study utilized RNA-seq technology and bioinformatics methods to characterize the gene expression profile of silver-blue mink muscle tissue. The results indicate that a total of 25,954 gene expression profiles were obtained during the growth and development stages of mink at 45, 90, and 120 days, with 2607 genes showing significant ...
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https://www.globenewswire.com/news-release/2024/04/19/2866242/0/en/Global-RNA-Analysis-Market-Forecast-2028-Rising-RNA-Seq-Data-Volume-Spurs-Demand-for-Advanced-Bioinformatics-Tools-Enhanced-Algorithms-Key-in-Harnessing-Genetic-Insights.html
Web5 days ago · The Global RNA Analysis Market has demonstrated a formidable rise, showing an increase in market valuation to USD 7.45 billion in 2022 and is anticipated to maintain a steady growth trajectory ...
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https://www.nature.com/articles/s41564-024-01655-4
Web3 days ago · Targeted accurate RNA consensus sequencing enables study of de novo errors caused by RNA-dependent RNA polymerases and provides deeper insights into how SARS-CoV-2 genetic diversity emerges.
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https://www.mdpi.com/2076-3921/13/5/506
Web1 day ago · This study employed RNA-Seq technology to elucidate the toxic effects of fluoride on ovine follicular GCs and its mechanism of action. Culturing primary ovine follicular GCs in vitro and subjecting them to fluoride treatment revealed 3218 differentially expressed genes (DEGs), with 2278 upregulated and 940 downregulated.
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https://www.ncbi.nlm.nih.gov/datasets/docs/v2/policies-annotation/genomeftp/
WebThe Genomes FTP site offers a consistent core set of files for the genome sequence and annotation products of all organisms and assembled genomes in scope. It supports download needs including: Retrieving the genome sequence for a specific assembled genome. Retrieving GenBank or RefSeq Gene, RNA, and protein annotation for a specific organism ...
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https://www.nature.com/articles/s43018-024-00756-7
WebApr 18, 2024 · We built PERCEPTION response prediction models for each drug in three steps. In step one, a bulk-expression model is trained to predict drug response in cell lines from the large-scale bulk-RNA-seq.
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https://www.nature.com/articles/s41467-024-47661-2
WebApr 18, 2024 · Direct RNA-seq offers the possibility to identify RNA modifications on single molecules. Here, the authors report on the synthesis of biologically realistic training data and the development of ...
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