Seahorse XF Glycolysis Stress Test Kit User Guide - Agilent
https://www.agilent.com/cs/library/usermanuals/public/XF_Glycolysis_Stress_Test_Kit_User_Guide.pdf
The XF instrument directly meas ures the acidification rate, and reports this as ECAR. The assay workflow is as follows. First, cells are incubated in the glycolysis stress test medium without glucose or pyruvate and the ECAR is measured. The first injection is a saturating concentration of glucose. The cells
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