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What are the principles of PCR?

Principle of PCR. The PCR involves the primer mediated enzymatic amplification of DNA. PCR is based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the offered template strand. Primer is needed because DNA polymerase can add a nucleotide only onto a preexisting 3′-OH group to add the first nucleotide.

What is the procedure of PCR?

PCR is an essential technique in cloning procedure which allows generation of large amounts of pure DNA from tiny amount of template strand and further study of a particular gene. The Human Genome Project (HGP) for determining the sequence of the 3 billion base pairs in the human genome, relied heavily on PCR.

What are the steps in a PCR reaction?

A polymerase chain reaction, or PCR, consists of three steps: DNA denaturation, primer annealing and extension. These steps are repeated between 20 and 35 times to synthesize the correct quantity of the DNA of interest.

What is a PCR protocol?

Protocols.io also provides an interactive version of this protocol where you can discover and share optimizations with the research community. PCR. The Polymerase Chain Reaction (PCR) is a powerful and sensitive technique for DNA amplification (1). Taq DNA Polymerase is an enzyme widely used in PCR (2).


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