Keyword Analysis & Research: colony pcr bacteria


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What is colony PCR and how does it work?

Colony PCR is a method used to screen for plasmids containing a desired insert directly from bacterial colonies without the need for culturing or plasmid purification steps. With SapphireAmp Fast PCR Master Mix, the colony PCR workflow is further improved and can be set-up with three basic steps: Pick a colony,

Can colony PCR be used for lactic acid bacteria?

According to this publication (see attached), yes, colony PCR can be used for Lactic acid bacteria. I believe that their PCR results would have been even prominent if they would have used less amount of colony for PCR (they used the whole colony to do PCR). Try and find out. Good luck. I agree with the less is better answer of Christian.

What is colon PCR and how does it work?

Colony PCR is a method used to screen for plasmids containing a desired insert directly from bacterial colonies without the need for culturing or plasmid purification steps. With SapphireAmp Fast PCR Master Mix, the colony PCR workflow is further improved and can be set-up with three basic steps:

What is the best lysis buffer for colony PCR?

An extremely simple and effective colony PCR procedure is established for both gram-negative and gram-positive bacteria, yeasts, and microalgae. Among the four lysis buffers examined, Y-PER is observed to be more effective than Tris/EDTA, 0.2 % SDS, and 10 mM EDTA in the extraction of PCR-quality ge …


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