3.6: cDNA - Biology LibreTexts
https://bio.libretexts.org/Bookshelves/Genetics/Working_with_Molecular_Genetics_(Hardison)/Unit_I%3A_Genes_Nucleic_Acids_Genomes_and_Chromosomes/3%3A_Isolating_and_Analyzing_Genes/3.06%3A_cDNA
WebJul 19, 2021 · a. First strand synthesis: First, one anneals an oligo dT primer onto the 3' polyA tail of a population of mRNAs. Then reverse transcriptase will begin DNA synthesis at the primer, using dNTPs supplied in the reaction, and copy the mRNA into complementary DNA, abbreviated cDNA.
DA: 59 PA: 85 MOZ Rank: 13