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How do you calculate cell concentration?

To calculate the initial concentration of the solution: Cell count per milliliter ÷ dilution factor = Cell concentration. For this example, the cell count per milliliter is 206,000, and dividing that by 10-2 (0.01) gives a cell concentration of 20,600,000 cells per milliliter in the initial sample.

What are the techniques of cell culture?

Among the common manipulations carried out on culture cells are media changes, passaging cells, and transfecting cells. These are generally performed using tissue culture methods that rely on aseptic technique. Aseptic technique aims to avoid contamination with bacteria, yeast, or other cell lines.


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