addgene gfp | Fluorescent Proteins 101: Green Fluorescent Protein (GFP

Keyword	CPC	PCC	Volume	Score	Length of keyword
addgene gfp	1.39	0.2	8258	20	11
addgene	1.97	0.6	5031	34	7
gfp	1.19	1	682	55	3

Keyword	CPC	PCC	Volume	Score
addgene gfp	1.28	1	2554	74
addgene gfp n1	0.48	0.9	1394	62
mscv ires gfp addgene	1.87	0.8	5612	93
gfp-luciferase plasmid addgene	0.3	0.1	6671	27
addgene anti gfp nanobody	1.68	1	7730	14
px458 gfp addgene	0.23	0.6	7249	60
cas9 gfp addgene	0.21	0.5	4220	1
gfp sequence addgene	1.99	0.8	3925	3

Search Results related to addgene gfp on Search Engine

Fluorescent Proteins 101: Green Fluorescent Protein (GFP) - Addgene
addgene.org

https://blog.addgene.org/plasmids-101-green-fluorescent-protein-gfp

WEBGFP is a ~27 kDa protein consisting of 238 amino acids derived from the crystal jellyfish Aequorea victoria. It has a fluorescent emission wavelength in the green portion of the visible spectrum (hence the name), which is due to a chromophore formed from a maturation reaction of three specific amino acids at the center of the protein (Ser65 ...

DA: 20 PA: 4 MOZ Rank: 99

Addgene: pCAG-GFP
addgene.org

https://www.addgene.org/11150/

WEBPlasmid pCAG-GFP from Dr. Connie Cepko's lab contains the insert EGFP from Aequorea victoria and is published in Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22. This plasmid is available through Addgene.

DA: 25 PA: 10 MOZ Rank: 86

Addgene: Fluorescent Protein Guide: Empty Backbones
addgene.org

https://www.addgene.org/fluorescent-proteins/plasmid-backbones/

WEBThe original green fluorescent protein (GFP) was cloned in 1992 ( Prasher et al., Gene, 1992 ), and since then scientists have engineered numerous GFP-variants and non-GFP proteins that result in a diverse set of colors. Addgene has assembled a collection of empty plasmid backbones with different fluorescent tags for you to create fusion ...

DA: 54 PA: 15 MOZ Rank: 73

Fluorescent Proteins 101: GFP Fusion Proteins - Making the ... - Addgene
addgene.org

https://blog.addgene.org/gfp-fusion-proteins-making-the-right-connection

WEBProtein size and shape matters. The green fluorescent protein (GFP) from Aequorea victoria and its variants are genetically encoded fluorescent probes. One of the limitations is the size of GFP: ~240 amino acids or about 28 kDa. GFP and its homologues have a beta-barrel structure.

DA: 86 PA: 19 MOZ Rank: 2

Addgene: GFP plasmids
addgene.org

https://www.addgene.org/browse/article/1613/

WEBAddgene is a nonprofit plasmid repository. We store and distribute high-quality plasmids from your colleagues.

DA: 30 PA: 86 MOZ Rank: 7

Fluorescent Proteins 101: When GFP lets you down - Addgene
addgene.org

https://blog.addgene.org/when-gfp-lets-you-down

WEBGFP is the most widely used fluorescent protein, but there are several instances that limits its use. GFP requires oxygen, is sensitive to acid, and its large size can be problematic. When thinking about ways to label proteins, a different probe may be more appropriate.

DA: 28 PA: 44 MOZ Rank: 84

Split Fluorescent Proteins for Studying Protein-Protein ... - Addgene
addgene.org

https://blog.addgene.org/split-fluorescent-proteins-for-studying-protein-protein-interactions

WEBMay 3, 2022 · Split super-folder GFP, our most-requested GFP(1-10) and GFP(11) Versatile protein tagging in cells with split fluorescent protein. Kamiyama et al. Nat Commun. 2016: Bo Huang: spGFP: Split superpositive GFP: Split-superpositive GFP reassembly is a fast, efficient, and robust method for detecting protein-protein …

DA: 85 PA: 67 MOZ Rank: 12

Multiplexed labeling of cellular proteins with split fluorescent
nature.com

https://www.nature.com/articles/s42003-021-01780-4

WEBFeb 26, 2021 · 13 March 2024. Introduction. In the self-complementing split GFP system, super-folder GFP is split between β-strands 10 and 11, rendering 214-amino acid and 16-amino acid fragments 1. The short...

DA: 19 PA: 39 MOZ Rank: 36

Plasmids 101: Protein tags - Addgene
addgene.org

https://blog.addgene.org/plasmids-101-protein-tags

WEBProtein tags are usually smallish peptides incorporated into a translated protein. As depicted in the accompanying cartoon, they have a multitude of uses including (but not limited to) purification, detection, solubilization, localization, or protease protection. Thus far Plasmids 101 has covered GFP and its related fluorescent proteins, which ...

DA: 49 PA: 78 MOZ Rank: 72

Plasmids 101: Knockout/Knock-In Plasmids - Addgene
addgene.org

https://blog.addgene.org/plasmids-101-knockout/knock-in-plasmids

WEBIf GFP is under control of an endogenous promoter, you can use expression GFP to track cells participating in development or other physiopathological events to which the chosen promoter responds. You can also use this method to tag an endogenous protein with GFP, as seen in blue flame plasmid OCT4-eGFP-PGK-Puro from the Jaenisch lab .

DA: 74 PA: 16 MOZ Rank: 36